STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to achieve an improvement in resolution, …

Jan 29, 2018 · Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical …

In STED microscopy, the specimen is illuminated by two synchronized ultrafast co-linear sources consisting of an excitation laser pulse followed by a red-shifted depletion laser pulse that is …

Stimulated Emission Depletion (STED) microscopy is a fluorescence microscopy super-resolution technique that is able to circumvent the optical diffraction limit. STED microscopy was first …

Researchers have used STED to image the fine structures within neurons, including the distribution of synaptic proteins and the organization of synaptic vesicles, which are tiny sacs …

STED microscopy uses two laser pulses to localize fluorescence at each focal spot. The first pulse is used to excite a fluorophore to its fluorescent state, and the second pulse is a …

The STED donut determines where the fluorescence originates in space; therefore, imaging with a single STED line ensures that the different channels (colors) are intrinsically aligned.

Stimulated emission depletion microscopy (STED) is a fluorescence microscopy technique that overcomes the diffraction limited resolution of confocal microscopes.

Nov 30, 2022 · STED (stimulated emission depletion) microscopy is a far-field super-resolution fluorescence microscopy technique that allows fluorescence imaging with, in principle, …

Jun 8, 2025 · In this series of articles on STED microscopy, I will cover more about how it works, optimising staining, and share some tips and tricks on how to get the most out of it.